Cast[]
- Yuri Lowenthal as Peter Parker/Spider-Man
- Nadji Jeter as Miles Morales/Spider-Man
- Laura Bailey as Mary Jane Watson
- Graham Phillips as Harry Osborn
- Jim Pirri as Sergei Kravinoff/Kraven the Hunter
- Tony Todd as Venom
- Griffin Puatu as Ganke Lee
- Jacqueline Pinol as Rio Morales
- Mark Rolston as Norman Osborn
- Mark Whitten as Curt Connors/Lizard
- Natasha Ofili as Hailey Cooper
- Leandro Cano as Flint Marko/Sandman
- Stephen Oyoung as Martin Li/Mister Negative
- Corey Jones as Lonnie Lincoln/Tombstone
- Tara Platt as Yuriko Watanabe/Wraith
- Chad Doreck as Cletus Kasady/Flame
- Erica Lindbeck as Felicia Hardy/Black Cat
- Ike Amadi as Aaron Davis
- Noshir Dalal as Quentin Beck/Mysterio
- Jason Spisak as Mac Gargan/Scorpion
- Nancy Linari as May Parker
- Russell Richardson as Jefferson Davis
- Darin De Paul as J. Jonah Jameson
- Ashly Burch as Danika Hart
- William Salyers as Otto Octavius/Doctor Octopus